Journal: bioRxiv

Article Title: Expression and role of Galectin-3 in the postnatal development of the cerebellum

doi: 10.1101/364760

Figure Lengend Snippet: (A) Lgals3 mRNA expression relative to Rpl13a measured by RTqPCR in the cerebellum during development. E17: embryonic day 17. P0, P7, P14, and P21: postnatal day 0, 7, 14, and 21. (B) Lgals3 knockout (KO) mice present no defects in cerebellar morphology. Parasagittal cerebellar slices of wild-type (WT) and Lgals3 KO adult mice were stained using the nuclear marker Hoechst.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-CABP (1:1000; swant, Cat#300), rabbit polyclonal anti-CABP (1:1000; swant, Cat#9.03), mouse monoclonal anti-GFAP (1:500; millipore, Cat#MAB360), rabbit polyclonal anti-GLURδ1/2 (1:1000; millipore, Cat#AB2285), goat polyclonal anti-LGALS3 (1:200; R&D Systems, Cat#AF1197), mouse monoclonal anti-OLIG2 (1:500; millipore, Cat#MABN50), guinea pig polyclonal anti-VGLUT1 (1:5000; millipore, Cat#AB5905) and guinea pig polyclonal anti-VGLUT2 (1:5000; millipore, Cat#AB2251).

Techniques: Expressing, Knock-Out, Staining, Marker

Journal: bioRxiv

Article Title: Expression and role of Galectin-3 in the postnatal development of the cerebellum

doi: 10.1101/364760

Figure Lengend Snippet: (A and B) Immunostaining for the Purkinje cell marker CABP (green) and the presynaptic marker VGLUT2 (red) was performed on sections from Lgals3 WT and KO mice at P15 (A) and at P65 (B). (C and D) Immunostaining for the Purkinje cell marker CABP (green) and the presynaptic marker VGLUT1 (red) specific for mature parallel fiber synapses was performed on sections from Lgals3 WT and KO mice at P15 (C) and at P65 (D). Scale bar: 25μm. Data are representative of three independent experiments.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-CABP (1:1000; swant, Cat#300), rabbit polyclonal anti-CABP (1:1000; swant, Cat#9.03), mouse monoclonal anti-GFAP (1:500; millipore, Cat#MAB360), rabbit polyclonal anti-GLURδ1/2 (1:1000; millipore, Cat#AB2285), goat polyclonal anti-LGALS3 (1:200; R&D Systems, Cat#AF1197), mouse monoclonal anti-OLIG2 (1:500; millipore, Cat#MABN50), guinea pig polyclonal anti-VGLUT1 (1:5000; millipore, Cat#AB5905) and guinea pig polyclonal anti-VGLUT2 (1:5000; millipore, Cat#AB2251).

Techniques: Immunostaining, Marker

Journal: bioRxiv

Article Title: Expression and role of Galectin-3 in the postnatal development of the cerebellum

doi: 10.1101/364760

Figure Lengend Snippet: (A) Immunohistochemistry for LGALS3 show the specificity of the LGALS3 antibody. WT: wild-type mice; KO: knockout mice. Positive cells in the molecular layer are marked with a white arrowhead, scale bar 50μm. (B) Immunohistochemistry for the Purkinje cell marker CABP (green) and LGALS3 (red) on parasagittal cerebellar sections from P15 and P22 wild-type mice. Scale bar=100μm. Data are representative of three independent experiments. Legends: 4V, 4 th ventricle; chp, choroid plexus; pcg, pontine central gray and mv, medial vestibular nucleus.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-CABP (1:1000; swant, Cat#300), rabbit polyclonal anti-CABP (1:1000; swant, Cat#9.03), mouse monoclonal anti-GFAP (1:500; millipore, Cat#MAB360), rabbit polyclonal anti-GLURδ1/2 (1:1000; millipore, Cat#AB2285), goat polyclonal anti-LGALS3 (1:200; R&D Systems, Cat#AF1197), mouse monoclonal anti-OLIG2 (1:500; millipore, Cat#MABN50), guinea pig polyclonal anti-VGLUT1 (1:5000; millipore, Cat#AB5905) and guinea pig polyclonal anti-VGLUT2 (1:5000; millipore, Cat#AB2251).

Techniques: Immunohistochemistry, Knock-Out, Marker

Journal: bioRxiv

Article Title: Expression and role of Galectin-3 in the postnatal development of the cerebellum

doi: 10.1101/364760

Figure Lengend Snippet: (A) LGALS3 expression in oligodendrocytes in the cerebellar white matter. Co-immunostaining of parasagittal cerebellar slices using anti-LGALS3 (red), oligodendrocyte marker anti-OLIG-2 (green) and the nuclear marker Hoechst (blue). (B) LGALS3 expression in astrocytes in the cerebellar white matter. Co-immunostaining of parasagittal cerebellar slices using anti-LGALS3 (red), astrocyte marker anti-GFAP (green) and the nuclear marker Hoechst (blue). (C) LGALS3 expression in some microglial cells of the molecular layer. Immunostaining anti-LGALS3 (red) in parasagittal sections of mice CX 3 CR1 eGFP/eGFP , microglial CX 3 CR1-GFP (green) and the nuclear marker Hoechst (blue). Scale bar 50μm and 20μm for the magnification. Data are representative of three independent experiments. Legends: 4V, 4 th ventricle; GL, granular layer; ML, molecular layer and WM, white matter.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-CABP (1:1000; swant, Cat#300), rabbit polyclonal anti-CABP (1:1000; swant, Cat#9.03), mouse monoclonal anti-GFAP (1:500; millipore, Cat#MAB360), rabbit polyclonal anti-GLURδ1/2 (1:1000; millipore, Cat#AB2285), goat polyclonal anti-LGALS3 (1:200; R&D Systems, Cat#AF1197), mouse monoclonal anti-OLIG2 (1:500; millipore, Cat#MABN50), guinea pig polyclonal anti-VGLUT1 (1:5000; millipore, Cat#AB5905) and guinea pig polyclonal anti-VGLUT2 (1:5000; millipore, Cat#AB2251).

Techniques: Expressing, Immunostaining, Marker

Journal: Frontiers in Cardiovascular Medicine

Article Title: Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia

doi: 10.3389/fcvm.2021.745810

Figure Lengend Snippet: Myeloid-specific Pfkfb3 deficiency attenuates LPS-induced inflammatory responses. (A) Representative images (left) and quantification (right) for immunohistochemical staining of the neutrophil marker Ly6G in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (B) Representative images (left) and quantification (right) for immunohistochemical staining of the macrophage marker Mac2 in lung sections of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 5). (C–E) qPCR analysis of the mRNA levels of Il1b (C) , Il6 (D) and Nos2 (E) in the lung of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 6). (F,G) ELISA analysis of Il1b (F) and Il6 (G) in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 4). (H) NO levels in serum of Pfkfb3 WT mice and Pfkfb3 ΔMϕ mice 6 h after LPS injection ( n = 3–4). All data are represented as mean ± SEM, ** P < 0.01 and *** P < 0.001 for Pfkfb3 WT vs. Pfkfb3 ΔMϕ (unpaired two-tailed Student's t test).

Article Snippet: After antigen retrieval with Antigen Unmasking Solution (H-3301, Vector Laboratories, Burlingame, CA, USA) at 98°C for 10 min, sections were blocked with avidin solution with 10% normal rabbit serum for 1 h at room temperature, and incubated in biotin blocking solution with primary antibodies against Mac2 (3 μg/mL, CL8942F, Cedarlane, Burlington, NC, USA), or Ly6G (3 μg/mL, 551459, BD biosciences, San Jose, CA, USA) at 4°C overnight.

Techniques: Immunohistochemical staining, Staining, Marker, Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test